How to set up Live Import

Live Import is a function that lets you import, convert and display 2D or 3D images in Aivia as they are being acquired on the microscope.

Requirements

• A microscope with attached acquisition hardware

• Aivia 7.5 or later

• A fast storage drive (such as an SSD) with sufficient storage space for Aivia internal cache plus full acquisition dataset

• Optional - A second fast storage drive for storing the full acquisition dataset

Step-by-step guide

1. Create a blank acquisition folder on your storage device for the experiment.

2. Point your acquisition software to save acquired images to the acquisition folder you just created.

3. Write down the expected image dimensions (X, Y, Z-slices, time points, number of channels and image bit-depth) for reference.

4. Go to Aivia and click on File > Start Live Import to launch the Live Import window

5. Click on the Browse icon to the right of the Import Folder textbox, select the acquisition folder in the Import Folder dialog, and click Select Folder.

6. In the Live Import window, enter the image dimensions for your acquisition into their respective fields.

7. Once you have verified that all the pertinent image-dimension information is correct, you can click Start to initiate Live Import mode in Aivia. 3D mode will be automatically enabled when you start Live Import for a an image with multiple Z-slices.

8. Begin your image acquisition protocol and start acquiring and saving images to the specified acquisition folder.