Aivia Software

Skip to end of metadata
Go to start of metadata

You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 6 Next »

You can add additional measurements to your analysis results (from recipe apply or manual edit) for additional object characterization using the Measurement Tool. The tool contains a number of pre-defined measurements and can be extended through the advanced measurements tab.


Launch measurement tool

The Measurement Tool can be found in the Spreadsheet tab in the Content panel at the bottom of the Aivia window. Clicking on the Measurement Tool  icon will launch a new modal window for selecting additional measurements on the current image. While the window is active, you will not be able to interact with the main GUI of Aivia.


Understand the measurement types

Aivia provides a plethora of measurement options depending on the objects that are available on the loaded image. You can find the measurement options that are available to you on the tabs at the top of the measurement dialog. Each measurement type correspond to a different type of analysis or different type of object.

There are twelve (12) types of measurements in Aivia:

Measurement TypeDescription
ObjectsMorphological and intensity measurements for individual 2D object outlines
Whole Image MaskMorphological and intensity measurements for 2D object outlines on the whole image as a single object
TracksTrajectory measurements, by individual time point as well as the whole trajectory, for each track objects
LineageMeasurements for lineage trees of tracked objects
MeshMorphological and intensity measurements for individual 3D object surfaces
NeuronMorphological measurements specifically for 3D neuron-type objects
SomaMorphological measurements specifically for 3D soma-type objects
DendriteMorphological measurements specifically for 3D dendrite-type objects
SpineMorphological measurements specifically for 3D spine-type objects, including spine head and spine neck subsets
RelationsMeasurements for characterizing the relationship between two object sets
ColocalizationMeasurements for characterizing the correlation between two image channels based on segmented regions (i.e. objects)
AdvancedCustom-defined measurements not included in the standard measurement types; please refer to the Advanced Measurement documentation for more information



On this page:


Select measurements

You can select a specific measurement by first navigating to the tab of the measurement type you would like to measure. Click on the checkbox next to the measurement description to select or deselect the measurement. You can also select/deselect all the measurements of a given type by clicking on the Select All or Deselect All button.


Measurement tabs and selection buttons

Measure another object set

When you have multiple object sets on your image, it is possible to specify the measurements to generate for each object set separately. First, click on the Object Set selection dropdown menu and select a different object set. The main measurement selection table will refresh to show the measurements that are selected for the given object set. Select the desired measurements from the table.

Measure on another channel

Certain object types (objects, whole image masks and meshes) allow you to specify the image channel to measure the intensity on. Recipe apply will measure the channel intensity of the input channel for the detected objects by default. Click on the Channel selection dropdown menu above the intensity measurement table and select a different channel. The intensity measurement table will refresh to show the selected intensity measurements for the given object set and the selected image channel. Select the desired measurements from the table.


Object set and channel selection dropdown menus

Rename

You can rename a measurement by double-clicking on the name of the measurement in the selection table. The name will be highlighted when rename is active. Enter a new name and press the Enter key on the keyboard to confirm.

Update

When you are finished with measurement selection, click the Finish button to close the Measurement Tool window and update the measurement list. All selected measurements will be calculated and available for review in the Spreadsheet tab or the Charts window.

To close the Measurement Tool without saving your selection or updating the measurements, click the Cancel button.




  • No labels