The Volume Transformer allows you to create a new version of an image that is rotated in 3D about any axis (X, Y, Z, or other) and has blank space from its edges trimmed, if that is opted for. You may adjust the angle of an ortho plane and/or choose a "bottom" plane to define the rotation angle.
The Volume Transformer is one of eight tools found in the 3D Tools tab in the Analysis Panel. Go to 3D View to show the 3D Tools tab. Click on the 3D Tools tab, and then click on the Volume Transformer icon to launch the tool. When the Volume Transformer is first launched, the GUI below appears.
Volume Transformer GUI |
Each time the Volume Transformer is launched, an ortho plane is shown. Any existing clipping planes are hidden, and an ortho plane is created if one did not exist prior to the launch of the tool.
Option Name | GUI Display | Description |
---|---|---|
Reset | Resets the transformation to its default state (no transformation) | |
Optimize Image Size | If selected, blank space will be trimmed from the sides of the transformed image, which can cause the transformation to be slower | |
Apply Transform | Applies the transformation, generating a new image that is added to the Workspace and displayed in the Image Viewer once it has been created |
You can access these options by clicking on the expand icon next to More Options.
Option Name | GUI Display | Description |
---|---|---|
Extra Alignment Points | Adds three points that are anchored to the ortho plane and can be moved parallel or perpendicular to the ortho plane, which can help with fine adjustment of the rotation angle | |
Show Intersecting Plane | Clips away the portions of the image that do not intersect with the ortho plane | |
Preview | Shows a preview of the transformation | |
Channels to Process | Allows you to select/deselect image channels to transform |
You can access this option by clicking on the expand icon next to More Options and then on the expand icon
next to Advanced.
Option Name | GUI Display | Description |
---|---|---|
Choose Bottom Plane | Allows you to... |
Using the Volume TransformerTo launch the Volume Transformer, click on the 3D Tools tab in the Analysis Panel in Aivia and select the Volume Transformer Align the Plane with the ImageSelect and drag the unidirectional red, green, and blue arrows to adjust the position of the alignment point; this can help ease the alignment process but will not affect the volume transformation. Select and drag the curved, double-sided red, green, and blue arrows to adjust the angle of the ortho plane; this will affect how the image is transformed. Align the plane so that it appears "flat" to the image.
Reset the Ortho PlaneClick Reset Use Extra Alignment PointsAdd three more alignment points to help with aligning the ortho plane to the image by selecting Extra Alignment Points Show the Intersecting PlaneSelect Show Intersecting Plane
Optimize Image SizeSelect Optimize Image Size Show a PreviewYou may turn on a preview by clicking the expand icon Select Image Channels to TransformBy default, the image channels that are visible are transformed, and those that are hidden are neither transformed nor included in the transformed image. Click on the expand icon
Choose a New Bottom PlaneTo change what the bottom plane (XY plane that appears furthest away when an image is first put in 3D View) will be in the transformed image, click on the expand icon Apply TransformClick Apply Transform |
Philipp Keller, Howard Hughes Medical Institute, Janelia Farms Research Campus, Ashburn VA; Cell Tracking Challenge, http://www.celltrackingchallenge.net/datasets.html