Aivia Software
Live import
Live Import is currently in Public Beta, please report any issues related to the usage of Live Import to Aivia.Support [at] leica-microsystems.com.
The Live Import function imports images (2D or 3D) as they are being acquired on a microscope and converts the file into Aivia's multi-resolution format for 3D display in real-time. The function looks for files being added into a user-specified acquisition folder and assembles the result sequence in Aivia. When acquisition is complete, you can save the compiled file in Aivia TIFF format without additional conversion steps.
To launch Live Import, go to File > Start Live Import.
On this page:
Interface
The Live Import dialog user interface is shown on the right. It has multiple options that let you specify the file dimensions and format. You can also choose to apply image fusion (for dual-view light sheet datasets) and add image calibration to the imported file.
Import parameters
The options in the Live Import dialog are as follows:
Name | Description |
|---|---|
Import Folder | Specifies the path to the acquisition folder |
Result Name | Specifies the name of the imported file |
Width | Specifies the width, or X dimension, of the acquired image in pixels |
Height | Specifies the height, or Y dimension, of the acquired image in pixels |
Z Planes | Specifies the Z dimension, or number of Z-slices in the acquired image |
Time Points | Specifies the time dimension, or number of total time points in the acquired image |
Channels | Specifies the number of image channels in the acquired image |
Acquisition Order | Specifies the acquired image layout, or organization of its dimensions The acquisition order can be as follows (first specified dimension to last specified dimension):
|
Bits Per Pixel | Specifies the bit-depth of the image; for 12- and 14-bit images, 16-bit is used |
Fusion | This option is available for importing single-channel images only. Applies fusion to blend acquired image volumes together - this is particularly important for dual-view light sheet microscopes where two image volumes are acquired per time point; you can specify the blending direction - along the X, Y, or Z axes - and choose from two blending modes:
|
X/Y Calibration | Specifies the lateral, or XY, resolution (unit per pixel) of the acquired image |
Z Calibration | Specifies the axial, or Z, resolution (unit per Z frame) of the acquired image |
Time Calibration | Specifies the temporal, or T, resolution (unit per time point) of the acquired image |
Controls
At the bottom of the dialog, there are six buttons:
Name | Description |
|---|---|
Simulator | Toggles the Live Import Simulator mode, which creates a simulated image sequence based on the parameters specified |
File | Toggles single-file import with incrementally appended data (see the "Supported formats" section of this page) |
Auto Seek | When enabled, the display will automatically jump to the most recently-converted image |
Apply the Current Recipe | Applies the recipe that currently appears in the Recipe Console to the images as they are imported |
Start | Starts Live Import mode (or with Simulator enabled, begins creation of the simulated image stack) |
Cancel | Closes the Live Import window without starting Live Import |
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Live Import window |