Parameters and Presets
Parameters
Recipe parameters for Particle Tracking and their descriptions are summarized in the table below.
Preset Group | Parameter Name | Min Value | Max Value | Description |
---|---|---|---|---|
Detection | Background Removal Factor | 0 | 100 | Adjusts the sensitivity of the background removal operation; a lower value will preserve larger objects and more background variations |
Object Enhancement Factor | 0 | 50 | Adjusts the sensitivity of the bright peak enhancement operation; a lower value will enhance smaller, individual peaks | |
Contrast Threshold | 0 | 255 | Adjusts the detection sensitivity on the background removed image; a lower value will detect more objects | |
Tracking | Motion vs Intensity | 0 | 10 | Adjusts the relative weighting between motion and object intensity for track-point matchmaking between successive frames; a value of 5 will apply equal weights to motion and intensity for matchmaking |
Gap Length | 0 | 10 | Specifies the maximum number of time frames for linking tracks that are spatially colocated; a higher value will preserve particle tracks for longer | |
Min Track Length | 0 | 50,000 | Specifies the minimum number of time frames before a detected object is considered a valid track; a lower value will generate more, and often shorter, tracks | |
Max Search Range | 0 | 50,000 | Specifies the maximum distance for track-point matchmaking between successive time frames; a higher value will expand the search distance for fast-moving cells |
Image credits
Laura Anne Lowery, Boston College, Boston MA; David Van Vactor, Harvard Medical School, Boston MA