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Object Set Settings is one (1) of the four (4) display settings sections. This section lets you adjust the appearance of each object set, as well as any additional overlay options, on the currently displayed image. The adjustments applied here only change how the image is shown in the Image Viewer and do not change the underlying data on the image.

Interface

The Object Set Settings panel is found in a column to the right of the Image Viewer below the Channel Settings by default. Each table row contains controls that applies to a particular object set or group. The object group display can look different for different types of objects.

Object group display

Below are examples of object group displays for different types of objects.

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titleOutlines (2D)

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titleTracks (2D)

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titleMeshes (3D)

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titleWhole Cells with Vesicle Spots (3D)

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titleNeurons (3D)

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titleTracks (3D)

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Buttons

The Create Object Set Template button Image Removed in the Object Set Settings table header opens a menu from which you can select to create an empty outline, mesh, or neuron set. You may choose to add objects created with the Outline Editor or 3D Tools to these sets, for example.

Each table row in the Object Set Settings panel represents a single object set on the displayed image. Each row can be controlled independently to adjust the display settings of each object set. There are six (6) basic controls for nearly every object set:

NameIconDescriptionToggle Visibility

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Shows or hides the object set from viewLoad Recipe Settings

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Loads the recipe settings and parameters used to generate this object set into the Recipe Console

Export Objects to a Mask

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Exports the objects to a mask; typical export options include the following:

  • 8-bit binary mask, which has an intensity of 255 for pixels/voxels contained by the objects and zero (0) for pixels/voxels outside of all objects in the set
  • 16-bit labelled mask, where pixels/voxels have intensities that correspond to the objects that contain them and have an intensity of zero (0) if they are outside of all objects in the set; 65535 unique object labels are available
  • 32-bit labelled mask, where pixels/voxels have intensities that correspond to the objects that contain them and have an intensity of zero (0) if they are outside of all objects in the set; 232-1 unique object labels are available

This button can also be used to export neuron groups to an Aivia-specific format.

Select Object Color Scheme

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Specifies the coloring mode for the object setSet Transparency

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Specifies the percent transparency of the object set in 3D ViewSelect Rendering Mode

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Specifies the rendering mode of the object set in 3D View; the available options are the following:

  • Solid Surfaces renders meshes as solid, closed surfaces
  • Wireframes renders meshes as sets of connected line segments
  • Points renders meshes as point clouds

Additional controls that are specific to particular object types are summarized in the table below.

Object TypeNameIconDescriptionDendritesSelect Dendrite Display Mode

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Specifies the display style for dendrite display, there are three (3) options:

  • Surface shows the dendrite as a full-formed surface
  • Sphere shows the dendrite as a thin trace connected by spheres with radii determined by the dendrite detection
  • Line shows the dendrite as a thin trace
Tracks, Spots
Set Overlay Radius

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Specifies the radius of the overlay display; this can be applied to tracks, anchors, trends, and spotsTracksSet Track Length

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Specifies the number of frames to display with the track overlay; a longer track length may result in reduced performanceShow/Hide Track Labels

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Shows/Hides track labels in Main View (2D)Select Anchor Display Mode

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Specifies the display style of the anchor overlay; there are two (2) options:

  • All shows all anchors on the displayed portion of a track
  • Current shows only the anchor for the current position of the track
Set Trend Display Mode

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Specifies the display style of the trend overlay; there are three (3) options:

  • Full Track shows the trend from the first to the final frame of a given track
  • First To Current shows the trend from the first frame to the current frame
  • Only Visible shows the trend from the earliest visible frame, based on the track length specified, to the current frame

Using the Object Set Settings

The Object Set Settings panel is active whenever you have an image loaded in Aivia. The software automatically populates the table based on the object sets and object types that are in the image file, if there are any.

Object sets can be organized into groups. Controls in a group row of of the Object Set Settings table often affect the settings for each object set in the group. The names of object groups and several types of object sets can be edited after their names are double-clicked in the Object Set Settings table.

Set display visibility

Each object set can be shown or hidden separately with the Toggle Visibility icon to the left of its name. The icon has two states:

Display StateIconDescriptionShown

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The associated item is visible; click on the icon to hide the associated itemHidden

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The associated item is not visible; click on the icon to show the associated item

For object groups, you can show or hide every object set and overlay in the group with the Toggle Visibility icon in the object group row.

Specify object display options that are unique to object types

The options available for displaying object sets and overlays depend on the object types. Please refer to the following pages for more information:

Change object set color

You can change the object set color display by clicking on the icon (or colored rectangle) associated with the object set in the Color column. Clicking on the icon will bring up a color picker menu which allows you to specify a different color or different coloring mode. There are seven (7) coloring options:

  • Solid color applies the same color to every object in the group
  • Color by label applies a different color to each object depending on their object label
  • Color by object type applies the same color to the same type of object (e.g. meshes, dendrites, spines)
  • Color by name applies a different color to each object depending on the name of the object
  • Color by measurements applies a different color to each object depending on its value for a specified measurement
  • Color by class applies the same color to objects that belong to the same classification group
  • Color by cell is specific to neuron and whole-cell objects and applies a different color for each neuron or whole cell

When you click on the icon in the Color column, the coloring option selector appears (see right). To change the coloring type, click on the dropdown menu in the selector.

You can also apply a specific color using the color picker below the dropdown menu. Click or drag the slider on the right to specify the desired hue (color) and click anywhere on the main display to specify the saturation and brightness desired. Alternately, you can specify a color by entering the hexadecimal color code in the textbox below the main display.

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Object color options

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Coloring option selector

Color objects by measurements

When you select the Color By Measurement option, a different coloring selector appears. You can specify the measurement you want to use as well as the color palette and measurement range. The Color By Measurement selector is shown on the right.

Click on Measurement to open a dropdown menu listing all the available measurements that can be used for this coloring mode. Click on a measurement name to select the measurement.

The minimum and maximum values are shown in the slider below the measurement selector. The values are automatically populated based on the minimum and maximum object measurements on the image. You can drag the slider to define the desired value range to color.

Below the value slider is the color palette selector, where you can specify a color gradient for coloring the measurements. Additionally, you can adjust the gradient by dragging each of the color markers or change the color using the color picker below.

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Color By Measurement selector

Delete object groups

You can delete an object group by right-clicking on its row and selecting Delete Group 

Object Settings is one (1) of the four (4) display settings sections. This section lets you adjust the appearance of each object set, as well as any additional overlay options, on the currently displayed image. The adjustments applied here only change how the image is shown in the Image Viewer and do not change the underlying data on the image.


Interface

The object settings display can be broken up into 3 sections, the Toolbar area, the Object Set Trees area, and the Display Settings area.

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Toolbar

The toolbar contains high level actions.

NameIconDescription
Collapse All

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Collapses all object sets in the tree view.
Expand All

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Expands all object sets in the tree view.
Create Object Set Templates

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Creates empty object set trees.

Opens a menu from which you can select to create an empty outline, mesh, or neuron set. You may choose to add objects created with the Outline Editor or 3D Tools to these sets, for example.

Copy Selected Objects

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If any objects selected, copies selected objects into a new object set structure like the existing one, an existing object set structure that's similar (e.g. neurons into neurons), or any mesh based objects into a standalone mesh object set.


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Object Set Tree View

The Object Set Tree View shows all object hierarchies, if any, on the image. The hierarchy of object sets represents different types of objects found on the image, such as Neurons, and all their components, like Somas, Dendrites, and Spines. It also contains any subsets associated with those object sets.

Object Set: Image Added

Object Subset: Image Added


You can control the visibility of any individual object set with the Image Addedbutton. The top level object set will toggle the visibility of all the object sets below it, as well as copy any display settings to all child object sets.

You can see the count for the set (n=Count) as well as the percentage of the parent object set. Note that the percentage pertains to the object set that that the subset is under and is not the percentage of the parent object subset (e.g. the percentage of the Affected subset on the right is based on the number of objects in Dendrite Set, and not the number of objects in the Treatment subset)

You can select an object set in this view, and more detailed settings will appear in the Detailed Display Settings area.


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Display Settings

In the display settings, you can see all the display settings for the object set or subset currently selected in the Object Set Tree View. Depending on what's selected, and what type of objects it contains, you may see different settings.

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titleOutlines 2D

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titleTracks 2D

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titleMeshes 3D

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titleDendrites 3D

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titleSubsets

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Buttons

Each table row in the Display Settings panel represents a single aspect of the currently selected object set or object subset. Each row can be controlled independently to adjust the display settings. There are six (6) basic controls for nearly every object set:

NameIconDescription
Toggle Visibility

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Shows or hides the object set from view
Load Recipe Settings

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Loads the recipe settings and parameters used to generate this object set into the Recipe Console. It's grayed out if the object set was not made using the recipe console. Only available on the top object set in an object set hierarchy.

Export Objects to a Mask

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Exports the objects to a mask or channel;  You may export to the following:

  • 8, 16, or 32 bit binary mask. The exported objects will have the maximum intensity for pixels/voxels contained by the objects and zero (0) for pixels/voxels outside of all objects in the set.
  • 8, 16, or 32 bit labelled mask.  The exported pixels/voxels have intensities that correspond to the objects that contain them and have an intensity of zero (0) if they are outside of all objects in the set.
    • 8 bit masks will allow a max of 255 unique objects labels.
    • 16 bit masks will allow a max of 65535 unique object labels.
    • 32 bit masks will allow a max of 2147483647 unique object labels.
  • 8, 16, or 32 bit binary channel. Same behavior as exporting to a binary mask, except the mask is added as a channel to the existing image. This is useful to convert an object set into an input image for training or recipe apply.
Select Object Color Scheme

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Specifies the coloring mode for the object set
Select Object Transparency

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Specifies the percent transparency of the object set.
Select Rendering Mode

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Specifies the rendering mode of the object set in 3D View; the available options are the following:

  • Solid Surfaces renders meshes as solid, closed surfaces
  • Wireframes renders meshes as sets of connected line segments
  • Points renders meshes as point clouds





Additional controls that are specific to particular object types are summarized in the table below.

Object TypeNameIconDescription
DendritesSelect Dendrite Display Mode

Image Added

Specifies the display style for dendrite display, there are three (3) options:

  • Surface shows the dendrite as a full-formed surface
  • Sphere shows the dendrite as a thin trace connected by spheres with radii determined by the dendrite detection
  • Line shows the dendrite as a thin trace
Tracks, Spots

Set Overlay Radius

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Specifies the radius of the overlay display; this can be applied to tracks, anchors, trends, and spots
TracksSet Track Length

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Specifies the number of frames to display with the track overlay; a longer track length may result in reduced performance
Show/Hide Track Labels

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Shows/Hides track labels in Main View (2D)
Select Anchor Display Mode

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Specifies the display style of the anchor overlay; there are two (2) options:

  • All shows all anchors on the displayed portion of a track
  • Current shows only the anchor for the current position of the track
Set Trend Display Mode

Image Added

Specifies the display style of the trend overlay; there are three (3) options:

  • Full Track shows the trend from the first to the final frame of a given track
  • First To Current shows the trend from the first frame to the current frame
  • Only Visible shows the trend from the earliest visible frame, based on the track length specified, to the current frame




Using the Object Set Settings

The Object Set Settings panel is active whenever you have an image loaded in Aivia. The software automatically populates the table based on the object sets and object types that are in the image file, if there are any.

Display settings in the top level object set in a tree often affect the settings for each object set in its hierarchy. The names of object sets  can be edited after their names are double-clicked in the Display Settings header or in the Object Set Tree View.

Set display visibility

Each object set can be shown or hidden separately with the Toggle Visibility icon to the left of its name. The icon has two states:

Display StateIconDescription
Shown

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The associated item is visible; click on the icon to hide the associated item
Hidden

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The associated item is not visible; click on the icon to show the associated item

For object trees, you can show or hide every object set in the group with the Toggle Visibility icon on top level object set.

Additionally, the right click menu has several options for quickly set the visibility of multiple object sets.

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  • Show all object sets will set the visibility of all object sets to visible.
  • Hide all object sets will set the visibility of all objects sets to hidden.
  • Show only "<Clicked Set>" will hide every set except the clicked on set.
  • Show only "<Clicked Set>" and its tree will set every child in the tree to visible and every other to hidden.
  • Show "<Clicked Set>and its tree will set all child sets in the tree to visible.
  • Hide "<Clicked Set> and its tree will set all child sets in the tree to hidden.

Specify object display options that are unique to object types

The options available for displaying object sets and overlays depend on the object types. Please refer to the following pages for more information:




Change object set color

You can change the object set color display by clicking on the icon (or colored rectangle) associated with the object set in the Color column. Clicking on the icon will bring up a color picker menu which allows you to specify a different color or different coloring mode. There are seven (5) coloring options:

  • Solid color applies the same color to every object in the group
  • Color by object type applies the same color to the same type of object (e.g. meshes, dendrites, spines)
  • Color by name applies a different color to each object depending on the name of the object
  • Color by measurements applies a different color to each object depending on its value for a specified measurement
  • Color by cell is specific to neuron and whole-cell objects and applies a different color for each neuron or whole cell

When you click on the icon in the Color column, the coloring option selector appears (see right). To change the coloring type, click on the dropdown menu in the selector.

You can also apply a specific color using the color picker below the dropdown menu. Click or drag the slider on the right to specify the desired hue (color) and click anywhere on the main display to specify the saturation and brightness desired. Alternately, you can specify a color by entering the hexadecimal color code in the textbox below the main display.




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Object color options










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Coloring option selector



Color objects by measurements

When you select the Color By Measurement option, a different coloring selector appears. You can specify the measurement you want to use as well as the color palette and measurement range. The Color By Measurement selector is shown on the right.

Click on Measurement to open a dropdown menu listing all the available measurements that can be used for this coloring mode. Click on a measurement name to select the measurement.

The minimum and maximum values are shown in the slider below the measurement selector. The values are automatically populated based on the minimum and maximum object measurements on the image. You can drag the slider to define the desired value range to color.

Below the value slider is the color palette selector, where you can specify a color gradient for coloring the measurements. Additionally, you can adjust the gradient by dragging each of the color markers or change the color using the color picker below.



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Color By Measurement selector



Coloring by Object Subsets

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In addition to the coloring options presented above there are some options for coloring a single object set to display which objects are part of which subset. These options can be found by right clicking an object set or object subset in the Object Set Tree View. Subsets being used for display will have the Image Addedicon next to them in the tree view. Emphasized sets/subsets will have their names bolded.

  • Spotlight affects the clicked on object set or object subset. It colors all objects that are in direct child subsets by their subset color, and any object not in a child subset gets colored by the parent object set/subset. If an object falls into more than one child subset, it will be colored white in 2D, and gray in 3D. In addition, all objects in the parent set well be emphasized.
  • Emphasize makes it so that objects that are emphasized stand out from non-emphasized objects. This is done by making non-emphasized objects much lighter and more transparent than emphasized objects. This currently only works on 2D object types.
  • Display using object set/subset color will override the display of any objects in that set to use the display color/mode of the set/subset you've selected. This provides a more granular display control over something like spotlighting.
  • Clear emphasis will remove the emphasis state on all objects in the field.
  • Clear display set will revert any spotlighting, emphasis, and revert to coloring objects just by their parent object set.

Spotlit dendrite set with subsetsImage Added

Delete object trees

You can delete an object tree by right-clicking on its row and selecting Delete Object Set(s) from the context menu. You will be prompted to confirm deleting the group. This action cannot be undone.




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