Object Set Settings is one (1) of the four (4) display settings sections. This section lets you adjust the appearance of each object set, as well as any additional overlay options, on the currently displayed image. The adjustments applied here only change how the image is shown in the Image Viewer and do not change the underlying data on the image.
Interface
The Object Set Settings panel is found in a column to the right of the Image Viewer below the Channel Settings by default. Each table row contains controls that applies to a particular object set or group. The object group display can look different for different types of objects.
Object group display
Below are examples of object group displays for different types of objects.
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Buttons
The Create Object Set Template button in the Object Set Settings table header opens a menu from which you can select to create an empty outline, mesh, or neuron set. You may choose to add objects created with the Outline Editor or 3D Tools to these sets, for example.
Each table row in the Object Set Settings panel represents a single object set on the displayed image. Each row can be controlled independently to adjust the display settings of each object set. There are six (6) basic controls for nearly every object set:
Loads the recipe settings and parameters used to generate this object set into the Recipe Console
Exports the objects to a mask; typical export options include the following:
- 8-bit binary mask, which has an intensity of 255 for pixels/voxels contained by the objects and zero (0) for pixels/voxels outside of all objects in the set
- 16-bit labelled mask, where pixels/voxels have intensities that correspond to the objects that contain them and have an intensity of zero (0) if they are outside of all objects in the set; 65535 unique object labels are available
- 32-bit labelled mask, where pixels/voxels have intensities that correspond to the objects that contain them and have an intensity of zero (0) if they are outside of all objects in the set; 232-1 unique object labels are available
This button can also be used to export neuron groups to an Aivia-specific format.
Specifies the rendering mode of the object set in 3D View; the available options are the following:
- Solid Surfaces renders meshes as solid, closed surfaces
- Wireframes renders meshes as sets of connected line segments
- Points renders meshes as point clouds
Additional controls that are specific to particular object types are summarized in the table below.
Specifies the display style for dendrite display, there are three (3) options:
- Surface shows the dendrite as a full-formed surface
- Sphere shows the dendrite as a thin trace connected by spheres with radii determined by the dendrite detection
- Line shows the dendrite as a thin trace
Specifies the display style of the anchor overlay; there are two (2) options:
- All shows all anchors on the displayed portion of a track
- Current shows only the anchor for the current position of the track
Specifies the display style of the trend overlay; there are three (3) options:
- Full Track shows the trend from the first to the final frame of a given track
- First To Current shows the trend from the first frame to the current frame
- Only Visible shows the trend from the earliest visible frame, based on the track length specified, to the current frame
Using the Object Set Settings
The Object Set Settings panel is active whenever you have an image loaded in Aivia. The software automatically populates the table based on the object sets and object types that are in the image file, if there are any.
Object sets can be organized into groups. Controls in a group row of of the Object Set Settings table often affect the settings for each object set in the group. The names of object groups and several types of object sets can be edited after their names are double-clicked in the Object Set Settings table.
Set display visibility
Each object set can be shown or hidden separately with the Toggle Visibility icon to the left of its name. The icon has two states:
For object groups, you can show or hide every object set and overlay in the group with the Toggle Visibility icon in the object group row.
Specify object display options that are unique to object types
The options available for displaying object sets and overlays depend on the object types. Please refer to the following pages for more information:
Change object set color
You can change the object set color display by clicking on the icon (or colored rectangle) associated with the object set in the Color column. Clicking on the icon will bring up a color picker menu which allows you to specify a different color or different coloring mode. There are seven (7) coloring options:
- Solid color applies the same color to every object in the group
- Color by label applies a different color to each object depending on their object label
- Color by object type applies the same color to the same type of object (e.g. meshes, dendrites, spines)
- Color by name applies a different color to each object depending on the name of the object
- Color by measurements applies a different color to each object depending on its value for a specified measurement
- Color by class applies the same color to objects that belong to the same classification group
- Color by cell is specific to neuron and whole-cell objects and applies a different color for each neuron or whole cell
When you click on the icon in the Color column, the coloring option selector appears (see right). To change the coloring type, click on the dropdown menu in the selector.
You can also apply a specific color using the color picker below the dropdown menu. Click or drag the slider on the right to specify the desired hue (color) and click anywhere on the main display to specify the saturation and brightness desired. Alternately, you can specify a color by entering the hexadecimal color code in the textbox below the main display.
Color objects by measurements
When you select the Color By Measurement option, a different coloring selector appears. You can specify the measurement you want to use as well as the color palette and measurement range. The Color By Measurement selector is shown on the right.
Click on Measurement to open a dropdown menu listing all the available measurements that can be used for this coloring mode. Click on a measurement name to select the measurement.
The minimum and maximum values are shown in the slider below the measurement selector. The values are automatically populated based on the minimum and maximum object measurements on the image. You can drag the slider to define the desired value range to color.
Below the value slider is the color palette selector, where you can specify a color gradient for coloring the measurements. Additionally, you can adjust the gradient by dragging each of the color markers or change the color using the color picker below.
Delete object groups
You can delete an object group by right-clicking on its row and selecting Delete GroupObject Settings is one (1) of the four (4) display settings sections. This section lets you adjust the appearance of each object set, as well as any additional overlay options, on the currently displayed image. The adjustments applied here only change how the image is shown in the Image Viewer and do not change the underlying data on the image.
Interface
The object settings display can be broken up into 3 sections, the Toolbar area, the Object Set Trees area, and the Display Settings area.
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Display Settings
In the display settings, you can see all the display settings for the object set or subset currently selected in the Object Set Tree View. Depending on what's selected, and what type of objects it contains, you may see different settings.
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Coloring by Object Subsets
In addition to the coloring options presented above there are some options for coloring a single object set to display which objects are part of which subset. These options can be found by right clicking an object set or object subset in the Object Set Tree View. Subsets being used for display will have the icon next to them in the tree view. Emphasized sets/subsets will have their names bolded.
- Spotlight affects the clicked on object set or object subset. It colors all objects that are in direct child subsets by their subset color, and any object not in a child subset gets colored by the parent object set/subset. If an object falls into more than one child subset, it will be colored white in 2D, and gray in 3D. In addition, all objects in the parent set well be emphasized.
- Emphasize makes it so that objects that are emphasized stand out from non-emphasized objects. This is done by making non-emphasized objects much lighter and more transparent than emphasized objects. This currently only works on 2D object types.
- Display using object set/subset color will override the display of any objects in that set to use the display color/mode of the set/subset you've selected. This provides a more granular display control over something like spotlighting.
- Clear emphasis will remove the emphasis state on all objects in the field.
- Clear display set will revert any spotlighting, emphasis, and revert to coloring objects just by their parent object set.
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